Isolation and identification of glyceryl monomycolate and of mycolic acid in phosphatide fraction of BCG.

In a chromatographic partitioning (Silicic acid-Hyflosupercel 2:1, Chloroform-methanol) of •gphosphatide•h of BCG, the author obtained three main peaks (Kondo, 1960) containing varied amounts of phosphorus. Glyceryl monomycolate was reported to distribute in neutral fat (Aoyagi et al., 1960) and in Wax A, C (Tsumita, 1956). It was assumed by the author that the glyceryl monomycolate can be migrated in this •gphosphatide•h fraction, because of its ubiquitous distribution among other fractions. The first peak of the chromatography (CHCl3-CH3OH 98:2) of •gphosphatide•h in one week as well as in 4 weeks culture was extracted with ether. Analytical data are